first molecular detection of chronic bee paralysis virus (cbpv) in iran
نویسندگان
چکیده
among the viruses infecting honey bees, chronic bee paralysis virus (cbpv) is known to induce significant losses in honey bee colonies. cbpv is an unclassified polymorphic single stranded rna virus. using rt-pcr, the virus infections in honey bees can be detected in a rapid and accurate manner. bee samples were collected from 23 provinces of iran, between july-september 2011 and 2012. a total of 160 apiaries were sampled and submitted for virus screening. rna extraction and rt-pcr were performed with qiagen kits. the primers lead to a fragment of 315 bp. the pcr products were electrophoresed in a 1.2 % agarose gel. following the rt-pcr reaction with the specific primers, out of the 160 apiaries examined, 12 (7.5 %) were infected with cbpv. this is the first study of cbpv detection in iranian apiaries. we identified cbpv in the collected samples from different geographic regions of iran.
منابع مشابه
First molecular detection of Chronic Bee Paralysis Virus (CBPV) in Iran
Among the viruses infecting honey bees, chronic bee paralysis virus (CBPV) is known to induce significant losses in honey bee colonies. CBPV is an unclassified polymorphic single stranded RNA virus. Using RT-PCR, the virus infections in honey bees can be detected in a rapid and accurate manner. Bee samples were collected from 23 provinces of Iran, between July-September 2011 and 2012. A tota...
متن کاملMolecular detection of Acute Bee Paralysis Virus in Iran
Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. The aim of this study was evaluation of ABPV infection in honey bee colonies ...
متن کاملMolecular identification and phylogenetic analysis of chronic bee paralysis virus in Iran
Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular ide...
متن کاملmolecular detection of acute bee paralysis virus in iran
acute bee paralysis virus (abpv) is a small single stranded rna virus recently classified within the family dicistroviridae, genus cripavirus. here, we describe the first study of abpv in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. the aim of this study was evaluation of abpv infection in honey bee colonies ...
متن کاملCharacterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry
Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA pol...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
archives of razi instituteناشر: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
دوره 70
شماره 4 2016
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023